A distinctive 'microbial signature' in celiac pediatric patients
Background:
Celiac Disease (CD) is an autoimmune disorder of the small intestine in which dietary gluten ingestion leads to a chronic enteropathy. Recently, scientific evidence suggested a potential role of gut microbiota in CD. To have a snapshot of dominant duodenal microbiota we analyzed the mucosa-associated microbiota of 20 children with CD, before and after a gluten-free diet (GFD) regimen, and of 10 controls. Total DNA was extracted from duodenal biopsies and amplification products of 16S ribosomal DNA were compared by temporal temperature gradient gel electrophoresis (TTGE). TTGE profiles were analyzed by statistical multivariate analysis.
Results:
The average number of bands in TTGE profiles was significantly higher (P (Source: BMC Microbiology - Latest articles)MedWorm Message: Register for MedMatcha, MedWorm's medical advertising network, and receive $5 free advertising.
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